Whitley i2 Instrument Workstation

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Whitley i2 Instrument Workstation

Designed for use with the Seahorse XF Analyzer

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The Whitley i2 Instrument Workstation has been developed in response to a rising number of enquiries from scientists who desire to use Seahorse Extracellular Flux (XF) Analyzers in hypoxic conditions and were dissatisfied with the solutions available. Rather than adapting an existing tissue culture incubator for this purpose, Don Whitley Scientific developed a new tissue culture incubator specifically to suit the precise requirements of Seahorse Bioscience.

Extensive research and careful consideration has gone into the development of the i2, which has resulted in a modular system that includes a number of novel and unique features. This workstation can be used as a stand-alone unit or connected to a Whitley Hypoxystation via the Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

This workstation is equipped with a generous working area in which to conduct preparatory work and is supplied complete with removable front, internal mains sockets, and a wireless footswitch to control the patented oval ports.

Another unique feature is the 4 litre integral incubator, a temperature controlled storage area that enables you to precondition cell ware and incubate plates/media at 37°C, under the same atmospheric conditions as the XF Analyzer.

If your research requires unsurpassed accuracy, reproducibility and reliability, then insist on a Whitley Workstation.

  • The i2 maintains an internal temperature no higher than 28°C, excludes carbon dioxide, and provides precise oxygen control.
  • The 12 litre airlock, with a cycle time of just 60 seconds, accommodates up to 7 x 0.5 litre bottles, 44 × 96 well plates or 84 × T25 tissue culture flasks plus numerous other flasks, pipettes and laboratory consumables.
  • The Whitley i2 Instrument Workstation is one of a range of larger Whitley Workstations developed to allow customers to use a variety of items of specialised equipment under modified atmospheric conditions.
  • The combination of a Whitley i2 Instrument Workstation and Seahorse XF Analyzer permits simultaneous, real-time analysis of mitochondrial respiration and glycolysis in mammalian cells under precisely controlled hypoxic conditions.

View Specification


Capacity (litres)
Airlock Capacity
Accommodates up to 44 x 96 well plates or 84 x T25s
Airlock Size (litres)
Porthole System
Manual Ports
Gas Supplies
Air and Nitrogen
Auto Sleeve Gassing
Internal Mains Sockets
Storage Tray
Inspection Lamp
Data Logging
Airlock Cycle Time (seconds)
Extra Cable Glands
Contact Us
Removable Front
Trolley Available
Vacuum Take Off
Temperature Range
<28°C (Main Chamber)
Touchscreen Control
Remote Access
Dimensions (W/D/H mm)
1702 / 825 / 928
Weight (lbs/kgs)
286 / 130
Internal Incubator
Whitley H35 Hypoxystation

Connect the i2 to an H35 HEPA for preparation of cell lines under hypoxic conditions

Whitley H35 Hypoxystation

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If you are based in Germany or Australia, please select the website of our subsidiary company there. A different selection of products is available in these countries. Otherwise, please select ‘English’ and you will be able to find your local distributor in the ‘Overseas Distributors’ section.

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