Whitley H45 Hypoxystation

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H45 3 OPS RF

A hypoxic workstation with a third port for instant access

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The H45 Hypoxystation provides the facility to mimic in vitro conditions, providing a continuous cell culture environment that eliminates cellular stress linked to variations in temperature, pH levels and oxidation, yielding better cell lines.

You can now control O2 in 0.1% increments up to 20%; CO2 in 0.1% increments up to 15%; and relative humidity up to 80%. This provides flexibility in your research and confidence in your results.

This hypoxic chamber can be fitted with three sleeved ports, two on a removable front, or one Instant Access Port and two sleeved ports. This workstation has sufficient space to accommodate a variety of equipment inside the chamber, whilst still providing a generous working and incubation area.

The H45 is also available as an H45 HEPA, equipped with the Whitley Internal HEPA Filtration System. All the atmosphere inside the chamber passes through the filter hundreds of times an hour, which ensure the chamber environment is cleaned quickly.

If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

Applications for this instrument include cancer research, IVF, stem cell research, cardiovascular research, as well as many other types of cell and tissue culture work.

An ideal solution for Seahorse Analyzer users is to purchase the H45 along with the new Whitley i2 Instrument Workstation. These two workstations can be connected via the Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

Equilibrate your media

Although the Hypoxystation allows tight control of the conditions under which cells are cultured, cells may still be unintentionally exposed to excess oxygen during media changes if the fresh media has not previously been equilibrated with the incubation atmosphere. With the Whitley Media Conditioner (UK Patent No. GB2579704) you can equilibrate your liquid media rapidly and ensure even greater accuracy in your research.

  • Three portholes provide convenient access to the entire incubation and working areas of the chamber.
  • The removable front facilitates thorough cleaning and the transfer of bulk samples or larger pieces of equipment for use in the workstation.
  • Built-in rapid airlock to ensure samples can be transferred into the workstation atmosphere as soon as possible (only takes 60 seconds).
  • Colour, touchscreen control panel for ease of use and for visual display of parameters such as O2 level, CO2 level, temperature, humidity and airlock cycle status.
  • Ethernet-enabled for remote access to touchscreen.
  • Available with fully automatic humidification system so you can add moisture AND maintain a sterile environment.
  • Fully integrated gas control avoids bulky add-on systems and reduces the need for additional bench space.

View Specification


Capacity (litres)
Airlock Capacity
Accommodates up to 44 x 96 well plates or 84 x T25s
Airlock Size (litres)
Porthole System
Instant Access or Manual Ports
Gas Supplies
CO2 / Air / N2
Auto Sleeve Gassing
Internal Mains Sockets
Storage Tray
Inspection Lamp
Single Sample Entry
Data Logging
Airlock Cycle Time (seconds)
Extra Cable Glands
Contact Us
Automatic Dehumidifier
Automatic Humidifier
Removable Front
Trolley Available
CO2 Monitoring
Vacuum Take Off
Temperature Range
5°C above ambient up to 45°C
Touchscreen Control
Dimensions (W/D/H mm)
1660 / 720 / 710
Weight (lbs/kgs)
295 / 134
Whitley H85 Hypoxystation

Need a larger airlock or a little more space - try the H85 Hypoxystation

Whitley H85 Hypoxystation

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If you are based in Germany or Australia, please select the website of our subsidiary company there. A different selection of products is available in these countries. Otherwise, please select ‘English’ and you will be able to find your local distributor in the ‘Overseas Distributors’ section.

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