New Scientific Paper: Isolation of Anaerobic Bacteria From the Digestive Tract of Chickens

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New Scientific Paper: Isolation of Anaerobic Bacteria From the Digestive Tract of Chickens

The University of Hohenheim, Germany recently published a scientific paper where they used a Whitley A35 HEPA Workstation in their research. Here is a brief summary of the work with a link to the full paper.

Title: Isolation of anaerobic bacteria from the digestive tract of chickens

Authors: Prof. A. Camarinha Silva, Prof. J. Seifert, University of Hohenheim, Institute for Livestock Sciences

Date: February 12, 2024

Description of the application

In the A35 HEPA Workstation (Don Whitley Scientific), fresh, anaerobically stored samples from the digestive tract of chickens are processed for the isolation of bacteria. This is done in a mixture of 80% N2 (quality level 5.0), 15% CO2 (quality level 3.0) and 5% H2 (quality level 5.0). The samples are serially diluted with a sterile physiological solution (0.85% NaCl). These dilutions were plated on different culture media and the isolates were separated over several weeks (Rios-Galicia et al 2024).


Whitley A35 HEPA Workstation

Special features required

  • A method of rapidly introducing fresh samples for further processing (A35 HEPA Workstation has an integral, 12 litre airlock).
  • An internal layout providing incubation space for Petri dishes for several weeks but with room to work (Whitley Workstation shelf system).
  • HEPA filter – because isolation of unknown, possibly pathogenic, bacteria could not be ruled out (Whitley Internal HEPA Filtration System).


Rios-Galicia B, Sáenz JS, Yergaliyev T, Roth C Camarinha-Silva A, Seifert J (2024) Novel taxonomic and functional diversity of eight bacteria from the upper digestive tract of chicken. International Journal of Systematic and Evolutionary Microbiology. 74(1): 006210. doi: 10.1099/ijsem.0.006210.

Additional publication

Rios Galicia B, Sáenz JS, Yergaliyev T, Camarinha-Silva A, Seifert J (2023) Host specific adaptations of Ligilactobacillus aviarius to poultry. Current Research in Microbial Sciences. 5. 100199. doi: 10.1016/j.crmicr.2023.100199.


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